method for introducing into the genome of a plant cell a target site for site, plant cell, plant part, plant or seed specific integration, method for integrating a polynucleotide of interest into a target site in the genome of a plant cell
E.I. DU PONT DE MOURS AND COMPANY;PIONNER HI-BRED INTERNATIONAL, INC.
发明人:
ANDREW MARK CIGAN,CHRISTOPHER JAY SCELONGE,DAVID J. PETERSON,HUIRONG GAO,LESZEK A. LYZNIK,MICHAEL W. LASSNER,SAVERIO CARL FALCO,ZHAN-BIN LIU,ZHONGSEN LI
申请号：
BR112014002622
公开号：
BR112014002622A2
申请日:
2012.07.18
申请国别(地区):
BR
年份:
2019
代理人:
摘要：
Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
