NG, Soon Chye,LIOW, Swee Lian,CHEN, Naiqing,TONG, Yen Wah,KHAN, Saif Abdul Kadir,FANG, Boon Sing
申请号：
SGSG2012/000031
公开号：
WO2013/115725A1
申请日:
2012.02.03
申请国别(地区):
WO
年份:
2013
代理人:
摘要：
According to embodiments of the present invention, a passive counter flow free microfluidic device for sorting a sample of flagellated cells is provided. The passive counter flow free microfluidic device includes an input reservoir configured to receive the sample of flagellated cells, an output reservoir positioned downstream of the input reservoir, the output reservoir configured to collect the flagellated cells that are sorted, and a plurality of microchannel sections arranged sequentially and in fluid communication with each other between the input reservoir and the output reservoir, and designed to define a closed microchannel system between the input reservoir and the output reservoir, wherein each microchannel section comprises at least one microchannel, wherein an inlet of the microchannel of a first microchannel section of the plurality of microchannel sections is connected to the input reservoir, wherein an outlet of the microchannel of a last microchannel section of the plurality of microchannel sections is connected to the output reservoir, and wherein, for each microchannel section, the total volume of the microchannels of one microchannel section of the plurality of microchannel sections is larger than that of the respective in said downstream direction subsequent microchannel section of the plurality of microchannel sections for sorting the flagellated cells when the flagellated cells move from the input reservoir to the output reservoir.Selon des modes de réalisation, la présente invention se rapporte à un dispositif microfluidique passif sans contre-courant destiné à trier un échantillon de cellules flagellées. Le dispositif microfluidique passif sans contre-courant comprend un réservoir dentrée configuré pour recevoir léchantillon de cellules flagellées, un réservoir de sortie positionné en aval du réservoir dentrée, le réservoir de sortie étant configuré pour collecter les cellules flagellées qui sont triées, et une pluralité de sections à micro-c
