There is provided a physiological detection device including a finger detection unit, a storage unit and a processing unit. The finger detection unit is configured to detect a current track drawn by a current user and current physiological information of the current user. The storage unit is configured to previously store track features of predetermined tracks drawn, for a predetermined time interval or a predetermined times on the finger detection unit, by a plurality of users and each of the track features is associated with one of the users. The processing unit is configured to analyze the current track and identify the current user according to the track features in the storage unit. There is further provided a physiological detection method and a user identification method.