PROBLEM TO BE SOLVED: To provide a highly bioadherent cultured periodontal ligament cell sheet having satisfactory adherence to a dental root surface.SOLUTION: A cultured periodontal ligament cell sheet that is manufactured by: culturing a cell under a specific condition on a cell culture support in which a substrate surface is coated by temperature-responsive polymer whose upper limit or lower limit critical dissolution temperature to water is 0 to 80°C (1) setting culture solution temperature to the upper limit critical dissolution temperature or higher, or the lower limit critical dissolution temperature or lower (2) closely adhering a cultured periodontal ligament cell sheet to a carrier and (3) peeling the sheet together with the carrier, has a ligament-like microstructure, extremely high bioadherence to a dental root surface, can transplant a target cell highly densely, and can positively reconstruct periodontal tissue. Furthermore, by laminating a cell sheet to be transplanted to allow the sheet to have three-dimensional polarity, an attachment organ can be reconstructed even more efficiently, and clinical application to middle-level periodontitis, serious periodontitis, gingival recession or the like is strongly expected.SELECTED DRAWING: NoneCOPYRIGHT: (C)2017,JPO&INPIT【課題】歯根表面への付着性が良好な高生着性培養歯根膜細胞シートを提供すること。【解決手段】水に対する上限もしくは下限臨界溶解温度が0~80℃である温度応答性ポリマーで基材表面を被覆した細胞培養支持体上で細胞を特定の条件下で培養し、(1)培養液温度を上限臨界溶解温度以上または下限臨界溶解温度以下とし、(2)培養した培養歯根膜細胞シートをキャリアに密着させ、(3)そのままキャリアと共に剥離することにより製造された培養歯根膜細胞シートは、靭帯様微小構造を有し、歯根表面への生着性が極めて高く、高密度に標的細胞を移植させられ、積極的な歯周組織の再建をはかれる。さらに、移植する細胞シートの積層化を行い3次元的な極性を持たせることにより、一層効率良く付着器官を再構築させることができ、中等度歯周炎、重度歯周炎、歯肉退縮等の臨床応用が強く期待される。【選択図】なし
