Barkan, Alice,Small, Ian,Rojas, Margarita,Bond, Charles,Fujii, Sota,Chong, Yee Seng
申请号：
AU2013248928
公开号：
AU2013248928A1
申请日:
2013.04.16
申请国别(地区):
AU
年份:
2014
代理人:
摘要：
A method of regulating expression of a gene in a cell is described, comprising the step of introducing into the cell a recombinant polypeptide comprising a PPR RNA-binding domain which itself comprises at least a pair of PPR RNA base-binding motifs. The PPR RNA base- binding motifs of the PPR RNA-binding domain are operably capable of binding the target RNA molecule with a target RNA sequence. Recombinant polypeptides comprising at least one PPR RNA-binding domain capable of binding to target RNA sequence are also described, together with fusion proteins comprising the recombinant PPR RNA-binding domains as well as isolated nucleic acids useful in preparing the recombinant polypeptides described. Recombinant vectors compositions comprising the recombinant polypeptides isolated nucleic acids recombinant vectors host cells comprising same use of same in the manufacture of a medicament for regulating gene expression as well as systems and kits for regulating gene expression are also described.
