ANIMAL PRODUCT-FREE CULTURE MEDIUM FOR BACTERIA OF THE GENUS CLOSTRIDIUM AND A PROCESS FOR PRODUCING SUPERNATANT COMPRISING ONE OR MORE COLLAGENOLYTIC AND GELATINOLYTIC PROTEASES
Marcos Castanheira ALEGRIA,Lucidio Cristovao FARDELONE,Marina Baiochi Riboldi DELALANA,Josef Ernst THIEMANN,Spartaco ASTOLFI FILHO,Roberto Carlos Debom MOREIRA,Ogari de Castro PACHECO
申请号：
US15650826
公开号：
US20180187144A1
申请日:
2017.07.14
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
