Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases
Marcos Castanheira Alegria,Lucidio Cristovao Fardelone,Marina Baiochi Riboldi Delalana,Josef Ernst Thiemann,Spartaco Astolfi Filho,Roberto Carlos Debom Moreira,Ogari de Castro Pacheco
申请号：
US14404155
公开号：
US09725692B2
申请日:
2013.05.29
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.
