Riken;Sumitomo Dainippon Pharma Co., Ltd.;Sumitomo Chemical Company, Ltd.
发明人:
申请号：
EP15852504.8
公开号：
EP3211072A1
申请日:
2015.10.23
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1) - (3):(1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state,(2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.
