Provided is a method for efficiently inducing differentiation from human pluripotent stem cells into hypothalamic neurons. Also provided is a method for constructing a cellular structure that integrates pituitary tissue and hypothalamic tissue from human pluripotent stem cells. A cellular structure including hypothalamic tissue is obtained by a method including a step for suspension culturing human pluripotent stem cell spheroids in a medium including a low concentration of an osteogenic factor signaling pathway activator and a low concentration of a substance capable of acting on the Shh signaling pathway, and a step for also suspension culturing the cell spheroids obtained in that step in a medium including a low concentration of a substance capable of acting on the Shh signaling pathway. A cellular structure including hypothalamic tissue and pituitary tissue is also obtained by a method including a step for suspension culturing human pluripotent stem cell spheroids in a medium including an osteogenic factor signaling pathway activator and a substance capable of acting on the Shh signaling pathway, a step for also suspension culturing the cell spheroids formed in that step in a medium including an osteogenic factor signaling pathway activator and a substance capable of acting on the Shh signaling pathway, and a step for suspension culturing the cell spheroids obtained in that step in a medium suitable for simultaneous pituitary and hypothalamic induction .La présente invention concerne un procédé dinduction de manière efficace de la différenciation de cellules souches pluripotentes humaines en neurones hypothalamiques. La présente invention décrit également un procédé de construction dune structure cellulaire qui intègre du tissu pituitaire et du tissu hypothalamique à partir de cellules souches pluripotentes humaines. Une structure cellulaire comprenant du tissu hypothalamique est obtenue selon un procédé comprenant une étape de culture en suspension de sphéroïde
