RIKEN;SUMITOMO CHEMICAL COMPANY LIMITED;SUMITOMO DAINIPPON PHARMA CO. LTD.
发明人:
申请号：
IL25185517
公开号：
IL251855D0
申请日:
2017.04.23
申请国别(地区):
IL
年份:
2017
代理人:
摘要：
The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1) - (3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGF² family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state, (2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.
