Sumitomo Chemical Company, Limited;Riken; Limited;Sumitomo Chemical Company
发明人:
Nakano, Tokushige,Sasai, Yoshiki,Ozone, Chikafumi
申请号：
AU2014345110
公开号：
AU2014345110A1
申请日:
2014.10.02
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
The present invention provides a method for producing retinal pigment epithelial cells, the method including: (1) a first step for forming an aggregate of pluripotent stem cells by suspension-culturing pluripotent stem cells in a serum-free medium； (2) a second step for suspension-culturing the aggregate formed in step (1) in a serum-free medium or serum medium including a BMP signal transduction pathway agonist and not including a sonic hedgehog signal transduction pathway agonist, and obtaining an aggregate including retinal precursor cells； and (3) a third step for suspension-culturing the aggregate obtained in step (2) in a serum-free medium or serum medium including a Wnt signal pathway agonist and not including a sonic hedgehog signal transduction pathway agonist or a BMP signal transduction pathway agonist, and obtaining an aggregate including retinal pigment epithelial cells.
