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PROCEDE DE PRODUCTION DE CELLULES EPITHELIALES DE PIGMENT RETINIEN
专利权人:
SUMITOMO CHEMICAL COMPANY, LIMITED;RIKEN
发明人:
NAKANO, TOKUSHIGE,SASAI, YOSHIKI (DECEASED),OZONE, CHIKAFUMI
申请号:
CA2930092
公开号:
CA2930092A1
申请日:
2014.10.02
申请国别(地区):
CA
年份:
2015
代理人:
摘要:
The present invention provides a method for producing retinal pigment epithelial cells, the method including: (1) a first step for forming an aggregate of pluripotent stem cells by suspension-culturing pluripotent stem cells in a serum-free medium; (2) a second step for suspension-culturing the aggregate formed in step (1) in a serum-free medium or serum medium including a BMP signal transduction pathway agonist and not including a sonic hedgehog signal transduction pathway agonist, and obtaining an aggregate including retinal precursor cells; and (3) a third step for suspension-culturing the aggregate obtained in step (2) in a serum-free medium or serum medium including a Wnt signal pathway agonist and not including a sonic hedgehog signal transduction pathway agonist or a BMP signal transduction pathway agonist, and obtaining an aggregate including retinal pigment epithelial cells.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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