There is provided an adhesion prevention film having excellent adhesive property and capable of improving adhesion effects on mucous membrane, which is manufactured by the method including processes of mixing an adhesion prevention composition, which includes distilled water, polyethylene oxide (PEO), and alginate, casting the mixed adhesion prevention composition and freeze-drying, to make a sponge-type film, compressing the sponge-type film, and irradiating one or more light sources selected from radioactive rays, electron beam, gamma rays, and ultraviolet rays on the compressed film to cross-link the film, in which a sponge-type film is first made by freeze-drying after casting the adhesion prevention composition, and then, the adhesion prevention film is made by cross-linking the freeze-dried film by compressing the film, thereby to provide the adhesion prevention film showing excellence in mechanical strength, flexibility, adhesive property, cytotoxicity, biodegradability, biocompatibility, adhesion prevention property, and the like.
