The present invention comprises the steps of obtaining rubusoside by pulverization of raw materials, flash extraction, double centrifugation, purification and desorption with a macroporous resin, enzymatic decomposition, classified decolorization with an organic membrane, concentration, crystallization, and drying. The present invention relates to a method for preparing rubusoside. According to the present invention, by optimizing the quality control of important processes such as extraction, separation, purification and decolorization, a rubusoside product with a purity of 99% or more is obtained, the product is pure white, has no bitterness, and has a residual solvent. There are few pesticide residues and product quality is stable.本発明は、原料の粉砕、フラッシュ抽出、二重遠心、マクロポーラス樹脂による精製及び脱着、酵素分解、有機膜による分級脱色、濃縮、結晶化、及び乾燥によりルブソシドを得るステップを含むことを特徴とするルブソシドの調製方法に関する。本発明によれば、抽出、分離、精製及び脱色などの肝要な工程の品質制御を最適化することで、純度99%以上のルブソシド製品が得られ、製品が純白で、苦味がなく、残留溶媒が少なく、残留農薬がなく、製品の品質が安定である。