Methods using a soft polysaccharide hydrogel for an organoid culture are described. An example method includes preparing a cell suspension in a cell culture medium. The cells of the cell suspension embedded or cultured in the 3D cell culture biomatrix are injectable for in vivo application. The method also includes mixing a hydrogel solution with the cell suspension to form a soft hydrogel mixture, adding additional cell culture medium to the soft hydrogel mixture to obtain cell colonies after a first time period, harvesting the cell colonies, mixing the hydrogel solution with the cell colonies to create a 3D cell culture biomatrix, adding cell differential medium to the hydrogel solution, replacing the cell differential medium with an organoid transfer medium after a second time period, and replacing the organoid transfer medium with an organoid medium after a third time period.
