HINDS, Kenneth,LEWIS, Danny,SCHMIDT, Paul,Campbell, Kathleen M
申请号：
DK12190040
公开号：
DK2599502T3
申请日:
2004.04.08
申请国别(地区):
DK
年份:
2017
代理人:
摘要：
The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB 1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.
