HINDS, KENNETH,LEWIS, DANNY,SCHMIDT, PAUL,CAMPBELL, KATHLEEN M.
申请号：
CA2521381
公开号：
CA2521381C
申请日:
2004.04.08
申请国别(地区):
CA
年份:
2020
代理人:
摘要：
The invention is directed to a single-step method for rapidly and efficientlypreparing protein-polymer conjugates, including an insulin-polymer conjugate.According to the method of the present invention, a protein and hydrophilicpolymer are contacted in the presence of at least one organic solvent and atleast one metal chelator, under conditions that promote the formation of aconjugate of the protein and polymer. Thus, the invention is directed to thesite-specific modification of selected proteins, such as insulin, withpoly(ethylene glycol) at residue PheB 1. The invention also provides apharmaceutical formulation for encapsulating the conjugate in a biodegradablepolymer.
