Kathleen M. Campbell;Danny Lewis;Paul Schmidt;Kenneth Hinds
发明人:
Kenneth Hinds,Danny Lewis,Paul Schmidt,Kathleen M. Campbell
申请号：
US10553570
公开号：
US09040664B2
申请日:
2004.04.08
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.
