The invention provides a printed circuit board and a detection method for detecting a connection between the printed circuit board and a flexible circuit board, and a display panel. The printed circuit board, through a connector provided thereon, is connected to a flexible circuit board, the printed circuit board is provided thereon with at least one first detection structure for matching with at least one second detection structure provided on the flexible circuit board, and whether or not a connection between the printed circuit board and the flexible circuit board is correct is determined by detecting matching between the first detection structures and the second detection structures. Pins of the printed circuit board may be correctly connected to pins of the flexible circuit board, and a case that short circuit occurs and thus the signal lines are damaged and burned can be avoided.
