PURPOSE: A shiitake mushroom medium manufacturing method is provided to use thermophilic fermentation with constant motion while cultivating and to manufacture a medium by inoculating a seed culture in a non-sterile room at 98 deg. C or 121 deg. C without sterilizing the medium.CONSTITUTION: A mushroom culture medium composition is put into a fermenter and controlled to have 60-65% moisture. The fermenter is operated to move the culture composition and is heated up to 75 deg. C while air is supplied in order to maintain an aerobic state. The culture composition is moved to maintain the temperature at around 70-75 deg. C by frictional heat and heat of decomposing thermophilic microorganisms. The culture is processed for 4-6 days by supplying air and evacuating the carbon dioxide. The temperature is rapidly decreased to 18-22 deg. C when the emission of the carbon dioxide from the culture composition is reduced. The culture composition is ejected from the fermenter and put into a plastic bag to be inoculated with the seed culture. The fermenter is continuously operated and controlled to take 2-3 minutes for one rotation after moisture is input until the inoculation is finished in order to move the culture composition. Air is supplied to the fermenter by a blower with 0.5 horse power per 3 tons of the culture composition after moisture is input until the inoculation is finished. The plastic bag has 20-50 holes with a diameter of 0.1-1 mm so that the shiitake mushroom hypha is able to breathe. After the medium is put into the plastic bag and the seed culture is inoculated, the entrance of plastic bag is sealed by heat or rolling the plastic bag without a stopper equipped with a filter.COPYRIGHT KIPO 2013본 발명은 유동고온발효에 의한 표고버섯 배지를 제조하는 방법에 관한 것으로, 배지 조성물을 계속 유동시켜서 마찰열과 미생물의 호흡열로 배지를 발효하여 유동고온발효 배지를 제조하는 것이다. 이렇게 하여 제조된 배지는 오염이 없기 때문에 종균접종을 무균실이 아닌 노출된 곳에서 접종이 가능하고 필터가 달린 뚜껑을 사용하지 않아도 되기 때문에 에너지와 노동력이 절감된다.
