PROBLEM TO BE SOLVED: To provide a method of culturing the mycelium of Sparassis crispa containing beta-glucan in a very short period of time.SOLUTION: The culture method of the mycelium of Sparassis crispa comprises the step of producing the inoculum by transplanting the fruit body of Sparassis crispa collected in the wild to an agar plate and culturing it, and inoculating the bacterial suspension to liquid culture medium dispensed in an Erlenmeyer flask and culturing it at constant temperature with shaking, the step of establishing grain culture medium adjusted to 4-6 in pH, the step of loading the grain culture medium into a PP bottle, the step of sterilizing the PP bottle containing the grain culture medium, the step of inoculating the inoculum, the step of culturing the mycelium inoculated to the grain culture medium in the condition of 20-30°C, humidity of 60-70% and CO2 indoor concentration of 2,000-2,500 ppm for 30-40 days while supplying oxygen, and the step of harvesting the cultured mycelium.COPYRIGHT: (C)2016,JPO&INPIT【課題】極めて短期間にベータグルカンを含有するハナビラタケ菌糸体を培養する方法の提供。【解決手段】野生で採取したハナビラタケ子実体を寒天平板培地に移植、培養し、菌液を三角フラスクに調剤された液体培地に接種し、恒温震盪培養する種菌製造段階と、pH4~6に調整して穀粒培地を造成する段階と、穀粒培地をPP瓶に入瓶する入瓶段階と、穀粒培地を含むPP瓶の殺菌段階と、種菌の接種段階と、穀粒培地に接種された菌糸を温度20~30℃、湿度60~70%、室内のCO2濃度2,000~2,500ppmの条件で30~40日間、酸素を供給しながら菌糸体を培養する段階と、培養された菌糸体を集菌する集菌段階とを含むハナビラタケ菌糸体の培養方法。【選択図】図1
