The invention provides a method of fabricating test strip for measuring biological fluid. The method comprises steps of: preparing a metallic membrane and an insulating material adhering the metallic membrane on the insulating material photolithographing for the metallic membrane etching the metallic membrane to form a circuit layer providing a sensing reagent on the circuit layer to form a sensing reagent layer and adhering an intermediate layer and a cover to the insulating material to cover the circuit layer and expose part of the circuit layer. The circuit layer is provided in an adhering manner to enhance the stability of resistance and the test accuracy.
