A test strip and a detecting method are disclosed. The test strip can be used with an electrochemical instrument to accurately detect the flow time of a specimen. The test strip includes a specimen path, a first electrode, a redox reagent, a second electrode, and a reaction reagent. The redox reagent includes at least a redox pair. When the specimen enters the specimen path, the redox pair dissolves and generates an electrochemical redox reaction for obtaining a flow time of the specimen.