An air quality test unit for attachment to a vent of a HVAC system with air flow there through. The air quality test unit contains at least two substrate panels arranged in a v-shape form wherein at least one panel includes a sticky surface or a collection container. The air quality test unit is attachable to the vent by one or more clips. The sticky surfaces or collection container of the substrate panels capture airborne substances and contaminants emanating from the air flow of the HVAC system vent. A culture growth medium may be disposed in the collection container or on the sticky surface. The air quality test unit kit provides a user with an air quality test unit and process for shipping the unit to the laboratory for analysis.