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MINI ANTIBODIES J591 AND DIGITAL DIAGNOSIS FOR DIRECTED DELIVERY OF HUMAN PROSTATE-SPECIFIC MEMBRANE ANTIGENE (PSMA) AND METHODS OF APPLICATION
专利权人:
ИМЭДЖИНЭБ; ИНК. (US)
发明人:
ХО Дэвид (US),ОЛАФСОН Тоув (US),ЛИПМАН Арие (US)
申请号:
RU2012123550/10
公开号:
RU2012123550A
申请日:
2010.12.02
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A mini-antibody encoded by a nucleotide sequence containing from the N-terminus to the C-terminus: a scFv sequence that can bind to a prostate-specific membrane antigen (PSMA), said scFv sequence contains a heavy chain variable domain (VH) linked to variable domain light chain (VL) linker sequence; artificial hinge region; and the sequence of human CH3 IgG. 2. The mini-antibody of claim 1, further comprising an N-terminal signal sequence that facilitates secretion of said mini-antibody when expressed in a cell. The mini-antibody of claim 1, wherein the scFv is located in a ba) VHVL orientation so that VH is located against the direction of transcription from VL; or b) VLVH orientation so that VL is located opposite the direction of transcription from VH.4. The mini-antibody according to claim 3, wherein the nucleotide sequence contains) SEQ ID No: 1 or SEQ ID No: 2; b) when expressed in a cell, the specified nucleotide sequence forms an amino acid sequence, and the specified amino acid sequence corresponds to SEQ ID No: 10 or SEQ ID No: 11; or c) when expressed in a cell, said nucleotide sequence forms an amino acid sequence, and wherein said amino acid sequence comprises: a heavy chain variable domain corresponding to a heavy chain variable domain in SEQ ID NO: 10 and a light chain variable domain corresponding to a light chain variable domain in SEQ ID no: 10; or the variable domain of the heavy chain corresponding to the variable domain of the heavy chain in SEQ ID No: 11 and the variable domain of the light chain corresponding to the variable domain of the light chain1. Мини-антитело, кодируемое последовательностью нуклеотидов, содержащее от N-конца к С-концу:последовательность scFv, которая может связываться с простата-специфичным мембранным антигеном (PSMA), указанная последовательность scFv содержит вариабельный домен тяжелой цепи (VH), соединенный с вариабельным доменом легкой цепи (VL) линкерной последовательностью;искусственный шарнирный участок; ипосле
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