Die Erfindung betrifft ein Verfahren zum Kryokonservieren von biologischem Material und eine Vorrichtung zum Durchführen des Verfahrens. Es ist die Aufgabe gestellt, eine Zellsuspension unter Erhalt der Lebensfähigkeit der Zellen ohne Kryoprotektiva einzufrieren. Die Aufgabe wird gelöst durch die Methode des Elektrospraying bei gleichzeitigen Minimieren der Folgen des Leidenfrostschen Phänomens.Cryopreserving biological material, comprises conveying a cell suspension at a flow rate of 10 ->3>-10 ->1>5>m 3>/second by a pump (3) to at least one nozzle (1), which is disposed opposite to an electrode (2) applying a direct current of 2-50 KV between the nozzle and electrode, which is produced by a high voltage generator (4) and introducing the formed droplets into a cryogenic vessel (7), which is located in the electric field of the electrodes and is filled with liquid nitrogen, such that the droplets are drawn further by the electric field to an anode. Cryopreserving biological material, comprises conveying a cell suspension at a flow rate of 10 ->3>-10 ->1>5>m 3>/second by a pump (3) to at least one nozzle (1), which is disposed opposite to an electrode (2) applying a direct current of 2-50 KV between the nozzle and electrode, which is produced by a high voltage generator (4) and introducing the formed droplets into a cryogenic vessel (7), which is located in the electric field of the electrodes and is filled with liquid nitrogen, such that the droplets are drawn further by the electric field to an anode with simultaneous deformation of a resulting Leidenfrost gas jacket and a very high cooling rates of the entire droplet content is produced, which includes the biological material. The droplets are discharged by colliding on an inclined cryogenic plate electrode, such that the Leidenfrost phenomenon is deactivated. The droplets are passed from the plate electrode into the cryogenic vessel, which is filled with liquid nitrogen, while an atmosphere is formed through the
