BIOTECHNOLOGY RESEARCH CENTER, SHANXI ACADEMY OF AGRICULTURAL SCIENCES;SUN, YI;YANG, LIYAN;DU, JIANZHONG;HAO, YAOSHAN;WANG, YIXUE;ZHANG, LIJUN;WANG, MING;YU, JIZHEN;ZHANG, TINGTING
Provided is a method ofultrasonic-aided Agrobacterium mediated gene transformation in plant germinated seeds, including the following steps: with the plant germinated seeds as receptor and an Agrobacterium Ti plasmid carrying an exogenous gene fragment as a gene vector, scarring a growth site of the plant germinated seed receptor and treating with ultrasonic in the process of seed germination, co-culturing with Agrobacterium; after the exogenous gene is transferred to a genome of the receptor, directly screening the seeds or seedling, and performing PCR amplification and Southem hybridization on DNA of the plant leaves, to further determine the transformed plant. In the method, the plant germinated seeds are directly used as the receptor for Agrobacterium infection, without tissue culturing and plant regeneration.
