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Method for improving Oryza sativa transformation efficiency using Agrobacterium-mediated transformation
专利权人:
发明人:
申请号:
KR20110046796
公开号:
KR101293654(B1)
申请日:
2011.05.18
申请国别(地区):
韩国
年份:
2013
代理人:
摘要:
PURPOSE: A method for transforming a plant using Agrobacteria is provided to remarkably improve transformation efficiency. CONSTITUTION: A method for trasforming a plant body using Agrobacteria comprises a step of removing albumen from the plant body and co-culturing the plant body with the Agrobacteria containing a recombinant expression vector for 7 or more days. The plant is cultured is cultured in an N6 meidum containing 1-3 mg/L of 2,4-dichlorophenoxyacetic acid at 25-32 Deg. C. for 4-7 days before removing albumen. The plant body is a callus of Oryza sativa. The Agrobacteria is Agrobacterium thumefacience strain LBA4404.
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