XISHUANGBANNA TROPICAL BOTANICAL GARDEN, CHINESE ACADEMY OF SCIENCES;XU, ZENGFU;PAN, JINGLI;FU, QIANTANG
发明人:
XU, ZENGFU,FU, QIANTANG
申请号：
WO2010CN01376
公开号：
WO2012019326(A1)
申请日:
2010.09.08
申请国别(地区):
世界知识产权组织国际局
年份:
2012
代理人:
摘要：
Provided is an Agrobacterium tumefaciens-mediated gene transformation method for Jatropha curcas, comprising the step of using cotyledons from Jatropha curcas seeds after cutting off 1/3-1/4 of the base of the cotyledons as explants for infecting with Agrobacterium tumefaciens, co-cultivating the cotyledon explants with Agrobacterium tumefaciens, then transfering onto the medium free of selective agent to induce callus for 4 weeks, subculturing onto the selection medium with the selective agent of kanamycin to select resistant shoots, subculturing the resistant shoots to obtain the resistant plants, and verifying the transgenic plants by molecular detection assay.
