PROBLEM TO BE SOLVED: To provide silica nanoparticles with encapsulated bioactive ingredients having a high function as drug delivery systems of the bioactive ingredients, and an efficient method of preparing the nanoparticles.SOLUTION: A silica nanoparticle comprises a multi-layered silica shells, where each shell has meso-pores and encloses a closed hollow space, optionally the innermost hollow closed space has a solid silica core; and one or more bioactive ingredients encapsulated within the hollow closed space, where the bioactive ingredients have size larger than the pore size of the shell encapsulating the bioactive ingredients, and where the bioactive ingredients in individual hollow spaces are identical or different.SELECTED DRAWING: None【課題】生物活性成分のドラッグデリバリシステムとして高い機能を有する生物活性成分内包シリカナノ粒子、及び前記ナノ粒子の効率的な調製方法の提供。【解決手段】シェルのそれぞれがメソ細孔を有し、閉じた中空空間を囲み、最も内側の前記閉じた中空空間は任意で固体のシリカコアをなす多層シリカシェルと;前記閉じた中空空間に内包された1つ以上の生物活性成分と;を含み、前記生物活性成分のサイズは、前記生物活性成分を内包する前記シェルの細孔径より大きく、前記中空空間のそれぞれにおける前記生物活性成分は同等又は異なる、シリカナノ粒子。【選択図】なし