Beach James M.,Lawrence Samuel M.,Cheatham Byron J.,Uertz James L.,Dougherty Robert P.
申请号：
US201414775309
公开号：
US10152823(B2)
申请日:
2014.03.12
申请国别(地区):
美国
年份:
2018
代理人:
Thomas Horstemeyer,LLP
摘要：
Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.
