The invention refers to a process for the in vitro regeneration of Mexican oregano (Poliomintha genus), which allows a complete plant to be obtained from an explant. The base vegetable explant is obtained from seed germination, apical meristem, stems, leaves or roots. The micropropagation is characterized in that it comprises the following steps: disinfection and in vitro establishment of the explants, induction of the callus formation for the indirect organogenesis with two phytoregulators BAP and NAA, regeneration of outbreaks (plants with stem and leaves) from the callus, obtention of plants with root from the outbreaks, acclimatization of the regenerated plants in a final substrate. The aforementioned process of the present invention acts in an efficient manner in the genotypes of wild species of Mexican oregano as Poliomintha Maderensis and Poliomintha Longiflora and Poliomintha Glabrescens Gray. This method allows sustainable agriculture schemes to be applied to this species, s afeguarding the genetic material and the diversity thereof, which is threatened with extinction, also providing a selective and distinguishable technique of the direct organogenesis for this species.La invención es un proceso de regeneración in Vitro de orégano mexicano (del género Poliomintha) el cual permite obtener una planta complete a partir de un explante. El explante vegetal base es obtenido a partir de germinación de semillas, meristemos apicales, tallos, hojas o raíces. El proceso de micropropagación está caracterizado porque comprende las siguientes etapas: desinfección los explantes y establecimiento in Vitro, inducción de la formación de callo para la organogénesis indirecta con dos fitorreguladores BAP y NAA, regeneración de brotes (plantas con tallo y hojas) a partir de callo, obtención de plantas con raíz a partir de brotes, aclimatación de las plantas regeneradas en sustrato final. El proceso descrito objeto de la presente invención funciona de manera eficiente en los ge
