LYNCH, GARY, S.,EVELETH, DAVID, D., JR.,SEUBERT, PETER, A.
申请号：
WO1991US04910
公开号：
WO9201935(A1)
申请日:
1991.07.11
申请国别(地区):
世界知识产权组织国际局
年份:
1992
代理人:
摘要：
The invention provides a method for the detection of cellular pathology by means of an immunoassay to determine the presence of stable breakdown products, termed BDP's or BDP1 and BDP2, of the cytoskeleton component spectrin. In one aspect of the invention, the components from a sample of spectrin-containing cells are physically separated, as by exposure to an electric field, in such a way that BDP and spectrin are separated. Antibodies reactive with BDP are then contacted with the separated sample, and their binding to that portion of the sample containing any BDP determined. In another aspect of the invention, an assay, such as an ELISA assay, is performed to detect total spectrin immunoreactivity as an indication of cellular death or degradation.
