The present invention provides a method for producing human ceramide in a yeast cell. The method of the present invention comprises: 1) introducing the sphingoid ”4-desaturase gene (DES1) by transformation of the yeast cell； 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell； and 3) abolishing the expression of the yeast sphingoid base kinase gene (LCB4) by transformation of the yeast cell.
