HARMON, Alexander M.,HARRIS, Ian Ross,KIHM, Anthony J.,MISTRY, Sanjay,MESSINA, Darin J.,SEYDA, Agnieszka,YI, Chin-Feng,GOSIEWSKA, Anna
申请号：
ES04777286
公开号：
ES2582342T3
申请日:
2004.06.25
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
Cells that can be obtained from human cord blood tissue substantially free of blood for use in inducing angiogenesis, wherein said cells are capable of self-renewal and differentiation, and have the following characteristics: a. potential to experience at least 40 duplications in the crop； b. binding and expansion in a coated or uncoated tissue culture vessel, wherein the coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyiorithin, vitronectin or fibronectin； C. production of vimentin and actin of smooth muscle alpha； d. production of each of CD10, CD13, CD44, CD73, HLA-A, B, C, CD90, HLA-A, B, C, PD-L2 and, PDGFralfa, as detected by flow cytometry； e) increased expression of endogenous genes encoding interleukin 8； reticulon 1； chemokine ligand 1 (motif C-X-C) (melanoma growth stimulating activity, alpha)； chemokine ligand 6 (motif C-X-C) (granulocyte chemotactic protein 2)； chemokine ligand 3 (motif C-XC)； and protein 3 induced by tumor necrosis factor alpha in relation to endogenous expression in a human cell that is a fibroblast, a mesenchymal cytoblast stem cell, or a bone marrow cell of the iliac crests, as characterized in a matrix gene； f) lack of production of CD31, CD34, CD45, CD80, CD86, CD 117, CD141, CD178, HLA-G, B7-H2 and HLADR, DP, DQ as detected by flow cytometry； g) growth of about 5% to about 20% oxygen h). require L-valine for growth； i) decreased expression of homeotic box 2 genes of short stature； 27 kDa heat shock protein 2； chemokine ligand 12 (motif C-X-C) (factor 1 derived from stromal cells)； elastin； DKFZp586M2022 cDNA (from clone DKFZp586M2022)； homeotic box 2 mesenchyme； homologue 1 of homeotic box of "sine oculis"； crystalline, alpha B； morphogenesis activator 2 associated with "disheveled"； DKFZP586B2420 protein； similar to neuralin 1； tetranectin； domain 3 homology with src (SH3) and rich in cysteines； B lymphocyte translocation gene 1, antiproliferative； cholesterol 25-hydroxylase； transcription fact
