MESSINA, Darin J.,MISTRY, Sanjay,HARMON, Alexander M.,HARRIS, Ian Ross,KIHM, Anthony J.,SEYDA, Agnieszka,YI, Chin-Feng,GOSIEWSKA, Anna
申请号：
ES04777235
公开号：
ES2568463T3
申请日:
2004.06.25
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
Cells that are obtainable from human umbilical cord tissue that are substantially free of blood for use in the treatment of a neurodegenerative condition, where cells are capable of self-renewing and expanding in culture, have the potential to differentiate into cells. of at least one neural phenotype, they require valine L for their growth, they can grow with about 5% to 20% oxygen, are multipotent or pluripotent and have the following characteristics: a. The potential to experience at least 40 dubbing in crops； b. They have adhesion and expansion in carriers of covered or uncovered tissue cultures, where the carrier of covered tissue cultures comprises a coverage of gelatin, laminin, collagen, polyomitin, vitronectin or fibronectin； C. They produce vimentin and alpha smooth muscle actin； d. They produce each of CD10, CD13, CD44, CD73, HLA-A, B, C, CD90, PD-L2 and PDGFr-alpha, as can be detected by means of flow cytometry； and. They have an increased expression of endogenous genes that code for interleukin 8； to crosslink 1； to chemokine ligand 1 (motif C-X-C) (melanoma growth stimulating activity, alpha)； to chemokine ligand 6 (motif C-X-C) (granulocyte chemotactic protein 2)； the chemokine ligand (motif C-X-C) 3； and tumor necrosis factor, the protein induced by alpha 3 in relation to endogenous expression in a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell, as can be analyzed by a oligonucleotide assay； F. They do not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, HLA-G, B7-H2 and HLA-DR, DP, DQ, as can be detected by flow cytometry； g. A reduced expression of the homeobox 2 of the short stature genes； Protein 2 from a 27kDa heat stroke； chemokine ligand 12 (motif C-X-C) (factor 1 derived from stromal cells)； elastin； DKFZp586M2022 cDNA (from clone DKFZp586M2022)； mesenchyme homeobox 2； homologue of homeobox 1 sine oculis； crystalline alpha B； scruffy associated activator of morphogenesis 2； DKFZP586B2420
