A method for vacuum ultrasonic low-temperature extraction of active ingredients in plants, which mainly includes the process of decomposing the crude plant fragments under anaerobic conditions with enzymes to break the walls, and then performing ultrasonic low-temperature extraction under low vapor pressure. With the combination of ultrasonic extraction technology and the method of enzyme wall breaking, while using enzymes to initially destroy plant cell walls, it is supplemented by ultrasonic waves to strengthen the decomposition of enzymes and improve the efficiency of enzymes to destroy cell walls. Furthermore, several different processes can be completed in series in the same extraction equipment, which can not only effectively shorten the extraction time, but also reduce the filtering residue processing time and the amount of solvent used, and avoid high temperature and oxidation to destroy the active ingredients, and maintain the activity of the extraction. The activity of the substance.一種真空超音波低溫萃取植物中活性成份之方法,主要係包含將經過粗碎之植物碎片,於無氧狀態下進行酵素分解破壁,再於低蒸氣壓下進行超音波低溫萃取。藉由超音波萃取技術與酵素破壁之方法的結合,於利用酵素對植物細胞壁進行初期破壞的同時,即輔以超音波強化酵素之分解作用,提高酵素破壞細胞壁的效率。進而可在同一個萃取設備內串聯並完成數個不同的製程,不僅能夠有效縮短萃取時間,也可減少過濾殘渣處理時間及溶劑使用量,並避免高溫及氧化作用破壞有效成分,保持萃取所得活性物質之活性。