The present disclosure pertains to the field of processing fresh traditional Chinese medicinal materials, and particularly relates to a method of preparing a fresh Ophiocordyceps sinensis product. The fresh Ophiocordyceps sinensis product may be prepared by the following steps: 1) stripping off mud from a fresh Ophiocordyceps sinensis; 2) brushing with flowing water; 3) cleaning ultrasonically; 4) humidifying the cleaned fresh Ophiocordyceps sinensis with atomized water for 15 to 20 minutes; 5) packaging the humidified fresh Ophiocordyceps sinensis by sealing it in a tube charged with a modified-atmosphere; and 6) storing the tube at a low temperature; wherein step 1) to step 4) are carried out at a temperature from 3 to 15° C. The present disclosure also relates to a fresh Ophiocordyceps sinensis product prepared by the above method.