SOCIETE DE COMMERCIALISATION DES PRODUITS DE LA RECHERCHE APPLIQUEE SOCPRA SCIENCES SANTE ET HUMAINES S.E.C.
发明人:
申请号：
EP03739915.1
公开号：
EP1529105B1
申请日:
2003.06.30
申请国别(地区):
EP
年份:
2012
代理人:
摘要：
The present invention relates to a method of modulating splice site selection, splicing and alternative, the method comprising the step of hybridizing an oligonucleotide-protein conjugate to a target pre-mRNA molecule in a cell or cell extract, wherein the oligonucleotide-protein conjugate comprises an oligonucleotide moiety which comprises at least two distinct sequence elements: (i) a nucleic acid sequence that is complementary to a specific region upstream of the splice site in the target pre-mRNA molecule； and (ii) an extension containing a protein binding site sequence element for covalently binding a protein； wherein the protein moiety comprises a protein capable of modulating splicing of the splice site upon binding with the protein binding site.
