Screening method for anticancer agent, and anticancer agent for inducing cell death, comprising as an active ingredient a substance that increases the activity of granzyme M
An object of the present invention is to provide a method for screening an anticancer agent using a novel therapeutic mechanism, and an anticancer agent for inducing cell death, which contains a substance that increases granzyme M activity as an active ingredient. is there. The method for screening an anticancer agent according to the present invention comprises a step of administering a test substance to a cancer cell, and a test substance whose activity has been confirmed by detecting the activity of granzyme M expressed by the cancer cell. And / or detecting the presence or absence of cancer cell death induced by activation of granzyme M expressed by cancer cells, and selecting a test substance in which cell death is confirmed； Have.本発明の目的は、新規な治療機構を利用した抗がん剤のスクリーニング方法、並びにｇｒａｎｚｙｍｅ Ｍの活性を増大する物質を有効成分とする、細胞死を誘導する抗がん剤を提供することである。本発明に係る抗がん剤のスクリーニング方法は、被検物質をがん細胞に投与する工程と、がん細胞が発現するｇｒａｎｚｙｍｅ Ｍの活性を検出し、活性の増大が確認された被検物質を選択する工程、及び／又は、がん細胞が発現するｇｒａｎｚｙｍｅ Ｍの活性化により誘導されるがん細胞の細胞死の有無を検出し、細胞死が確認された被検物質を選択する工程と、を有する。
