The present invention discloses a submerged cultivation method ofPleurotus eryngiimycelia with high ergothioneine (EGT) content. The present invention further providesPleurotus eryngiimycelia obtained by the cultivation method. ThePleurotus eryngiimycelia of the invention is useful in large-scaled liquid fermentation of ergothioneine, and can be used in the production ofPleurotus eryngiifruit body with high EGT content.本發明係關於高麥角硫因含量之杏鮑菇菌絲體的液態培養方法。本發明亦關於以該培養方法獲得之高麥角硫因含量杏鮑菇菌絲體培養物。該菌絲體培養物可用於液態發酵生產麥角硫因(ergothioneine),及進一步用於固態接種培養高麥角硫因含量之杏鮑菇子實體。