Disclosed is a preparation containing ergothioneine, the ergothioneine is provided by an extracellular ferment liquor which is obtained by removing the mycelia from the ferment liquor produced through liquid fementation of a mushroom and/or the concentrate of the extracellular ferment liquor, and the preparation comprises food, cosmetics and animal feed. The method for preparing the preparation comprises liquid fermentation of a mushroom, removing the mycelia from the fermentation product, and selectively concentrating the extracellular ferment liquor obtained after removing the mycelia. The extracellular ferment liquor obtained through the liquid fermentation of a mushroom and/or the concentrate of the extracellular ferment liquor are used in preparing the preparation containing ergothioneine.
