Process, vectors and engineered cell lines for enhanced large-scale transfection

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专利权人:

NATIONAL RESEARCH COUNCIL OF CANADA

发明人:

Durocher, Yves,Loignon, Martin

申请号：

EP20120192258

公开号：

EP2631297(B1)

申请日:

2009.03.09

申请国别(地区):

欧洲专利局

年份:

2017

代理人:

摘要：

An expression system for stable expression of a gene of interest is described, the expression system comprising: a first vector having an Epstein-Barr virus nuclear antigen-1 (EBNA1) nucleotide sequence encoding a truncated EBNA1 protein, a promoter and a polyadenylation signal for the EBNA1 nucleotide sequence and an oriP nucleotide sequence; and, a second vector having a gene of interest, a promoter and a polyadenylation signal for the gene of interest and an oriP nucleotide sequence.

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