The present invention relates to the LPS of neissers coccus to have six acylated lipid A parts wherein six acylated lipid A moieties are compared the wild type neissers coccus LPS of lipid A moiety is had modified, because it includes the non-reducing end lipid A moiety that palmitoleoyl replaces the gucosamine of secondary lauroyl chain. The invention additionally relates to the mixtures of six acylated LPS to lack the non-reducing end lipid A moiety of the gucosamine of secondary acyl chain with the acylated LPS of corresponding pentachlorophenol. It is to reduce to express the endogenous lpxL1geneand to the heterologous temperature-sensitive lpxP genes generation six and the acylated LPS of pentachlorophenol for introducing expression by genetic modification the invention further relates to the bacterium of neissers coccus. Pass through selection time and/or temperature when bacterial growth it is possible that the acylated lipid A class formation of the six of the amount of increasing or decreasing adjusts the neissers coccus of the LPS of TLR4 agonist activities, the method for exact level activity of the invention for specific immunization therapy relative to the acylated structure of corresponding pentachlorophenol.
