An aqueous solution containing ozone nanobubbles that can not only stably maintain high bactericidal or antibacterial activity due to ozone, but can also significantly reduce invasiveness to cells in living tissue, a method for producing the same, and use of the aqueous solution containing ozone nanobubbles I will provide a. The aqueous solution containing ozone nanobubbles of the present invention has an average particle diameter and density of ozone nanobubbles of 30 nm or less and 1016 or more per ml, preferably 1 to 10 nm, as measured with a cryo-transmission electron microscope by the ice embedding method. 1017 or more per ml. The aqueous solution containing ozone nanobubbles of the present invention is an aqueous solution containing dissolved ozone injected from the outside of a cylinder having two or more through-holes in the circumferential direction through a through-hole at a pressure equal to or higher than atmospheric pressure, and a radial cross section of the cylinder And ozone nanobubbles generated by colliding so that water hammer concentrates on the center of the cylinder on the same plane parallel to the surface.オゾンによる高い殺菌力又は抗菌力が安定的に持続できるだけでなく、生体組織内の細胞等への侵襲性を大幅に低減できるオゾンナノバブルを含む水溶液とその製造方法及び前記オゾンナノバブルを含む水溶液の使用を提供する。本発明のオゾンナノバブルを含む水溶液は、氷包埋法によってクライオ透過型電子顕微鏡で測定したときのオゾンナノバブルの平均粒径及び密度がそれぞれ３０ｎｍ以下及び１ｍｌあたり１０１６個以上、好ましくは１～１０ｎｍ及び１ｍｌあたり１０１７個以上である。本発明のオゾンナノバブルを含む水溶液は、溶存オゾンを含む水溶液を、２以上の貫通小穴を周方向に有する筒の外部から該貫通小穴を通して大気圧以上の圧力で噴射させ、前記筒の径方向断面と平行な同一平面上で前記筒の中心に水撃が集中するように衝突させて発生させたオゾンナノバブルを含む。
