The present invention provides a method of preparing antibacterial lotion, which includes providing a monomer mixture including three different monomers, each having a functional group different from each other providing an emulsifying agent water solution and an antibacterial agent water solution mixing the monomer mixture, the emulsifying agent water solution, the antibacterial agent water solution, a buffering agent and an initiator to form an antibacterial material water solution and performing an emulsion polymerization reaction at a predetermined temperature so as to form the antibacterial lotion of the present invention. When using the method of preparing antibacterial lotion in accordance with the present invention and its application to prepare modified active carbon, the modified active carbon is provided with advantages of extreme decrease of the dissolution amount of fine powders, excellent antibacterial material adsorbing control, excellent antibacterial capability, excellent active carbon adsorbing capability, and no residual monomer.本發明提供一種製備抗菌乳液之方法,其係包括提供一單體混合物,該單體混合物包含有三種不同單體,各單體具有一與彼此不相同之官能基;提供一乳化劑水溶液以及一抗菌劑水溶液;以及混合前述之單體混合物、乳化劑水溶液、抗菌劑水溶液、一緩衝劑以及一起始劑,以形成一抗菌材料水溶液;以及於一溫度下進行乳化聚合反應,以形成本發明之抗菌乳液。本發明之製備抗菌乳液之方法及其應用在製備改質之活性碳時,改質之活性碳能具備微細粉溶出量大幅下降、優良的抗菌材料吸附控制、優良的抗菌能力、優良的活性碳吸附能力以及無單體殘留等多項優點。