De Boer Anne Douwe,Ebskamp Michaël Johannes Marcus,Langeveld Simon Albertus,Laros Ivo,Van De Rhee Miranda Debora
申请号：
US200912933083
公开号：
US9695469(B2)
申请日:
2009.03.17
申请国别(地区):
美国
年份:
2017
代理人:
Morrison & Foerster LLP
摘要：
The invention relates to a method for analyzing a genomic region of an organism, comprising four major parts. The first part involves the isolation of mRNA from a selected organism that is used for the preparation of small single stranded DNA fragments with one adaptor containing an affinity label. These DNA fragments are used in part three. In the second part, genomic DNA from the same or a related organism is isolated. This genomic DNA is fragmented and ligated to adaptor molecules. In the third part, these genomic fragments are hybridized with single stranded DNA fragments from part one, and the hybrids formed in this process are used for synthesis of DNA fragments. These fragments will be used in part four which involves sequencing of these fragments using one of the available high throughput sequencing methods.
