A trace evidence material (TEM) collection device is disclosed that enables crime scene investigators to quickly and easily collect, analyze, annotate, securely store and electronically distribute images of large amounts of trace evidence materials and related crime scene information while also helping to comply with required trace evidence recovery procedures and documentation requirements. The TEM collection device includes a reusable handle and cassette drive mechanism, which may be used with a plurality of single-use cassettes. Each cassette includes a TEM collection media (such as a collection tape or swabbing pads attached to a substrate) that when moved across a surface is capable of collecting TEMs located on the surface. Preferably, each cassette also includes a sealing assembly that seals the TEM collection media after collection of the TEMs so as to preserve the collected TEMs.