The present invention relates to a nanofibers tissue engineering scaffold comprising mussels coating proteins and Fe3+ ions and a manufacturing method thereof. More specifically, the present invention relates to a nanofibers tissue engineering scaffold which the surface of the nanofibers is coated by bioactive substances, the mussels adhesive proteins is a nanofibers tissue engineering scaffold which more than 20% of the total tyrosine residues are modified to DOPA.본 발명은 홍합 접착 단백질 및 Fe3+ 이온을 포함하는 나노 섬유 조직공학용 지지체(scaffold) 및 이의 제조방법에 관한 것이다.