您的位置: 首页 > 农业专利 > 详情页

Proces lizy/ponownego zamykania do wprowadzania substancji czynnej do erytrocytów
专利权人:
Erytech Pharma
发明人:
GODFRIN, YANN
申请号:
PL05768180
公开号:
PL1773452T3
申请日:
2005.08.04
申请国别(地区):
PL
年份:
2012
代理人:
摘要:
Lysis/resealing process for preparing erythrocytes which contain an active ingredient, the process comprising the following steps: (1) - placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65 %, with refrigeration at from + 1 to + 8 °C, (2) - measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension obtained in step (1), (3) - lysis and internalisation procedure of the active ingredient, inside the same chamber, at a temperature which is constantly maintained at from + 1 to + 8 °C, comprising allowing the erythrocyte suspension having a haematocrit level which is equal to or greater than 65 % and a hypotonic lysis solution which is refrigerated at from + 1 to + 8 °C, to circulate in a dialysis cart ridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and (4) - resealing procedure carried out in a second chamber at a temperature of from + 30 to + 40 °C by means of a hypertonic solution.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

意 见 箱

匿名:登录

个人用户登录

找回密码

第三方账号登录

忘记密码

个人用户注册

必须为有效邮箱
6~16位数字与字母组合
6~16位数字与字母组合
请输入正确的手机号码

信息补充